14075-00-4 → HCID 86586587, compound

HRID 1823248Notes

Reaction equation

HRID 1823248 reaction equation
[Cl:1][C:2]1[N:10]=[CH:9][N:8]=[C:7]2[C:3]=1[NH:4][CH:5]=[N:6]2.P([O:15][C@H:16]1[O:22][C@H:21]([CH2:23][OH:24])[C@@H:19]([OH:20])[C@H:17]1[OH:18])(O)(O)=O>>[Cl:1][C:2]1[N:10]=[CH:9][N:8]=[C:7]2[C:3]=1[N:4]=[CH:5][N:6]2[C@:16]1([O:22][C@H:21]([CH2:23][OH:24])[C@@H:19]([OH:20])[C@H:17]1[OH:18])[OH:15]

Participants

3

Reactant 2

HCID 43923614075-00-4HCID 53592776-Chloropurine

Product 1

HCID 86586587HCID 86586587, compound

Procedure

One mL of a reaction solution consisting of 10 mM 6-chloropurine (Aldrich), 50 mM D-ribose-1-phosphate (22) prepared in Example 10, 0.1 mL of the ultrasonic enzyme homogenate from a purinenucleoside-phosphorylase producing strain prepared in Example 25 and 100 mM Tris-hydrochloride buffer (pH 7.5) was reacted at 50° C. for 20 hours. After completion of the reaction, HPLC analysis for the reaction solution under the conditions described below showed a peak of the title compound. A reaction inversion rate was calculated to be 62.4% after determining the concentration of 6-chloro-9-(β-D-ribofuranos-1-yl)purine in the post-reaction solution.

Workup

  1. customwas reacted at 50° C. for 20 hours
  2. customAfter completion of the reaction, HPLC analysis for the reaction solution under the conditions

Sources & evidence

ORD record
ord-b2b687ce754848f49e95daf5330d7c82
Source dataset
uspto-grants-2006_05
Patent number
US07038039B2

Reaction notes

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