Ribose-1-Phosphate → HCID 86586587, compound

Participants

Reaction Steps

One mL of a reaction solution consisting of 10 mM 6-chloropurine (Aldrich), 50 mM D-ribose-1-phosphate (22) prepared in Example 10, 0.1 mL of the ultrasonic enzyme homogenate from a purinenucleoside-phosphorylase producing strain prepared in Example 25 and 100 mM Tris-hydrochloride buffer (pH 7.5) was reacted at 50° C. for 20 hours. After completion of the reaction, HPLC analysis for the reaction solution under the conditions described below showed a peak of the title compound. A reaction inversion rate was calculated to be 62.4% after determining the concentration of 6-chloro-9-(β-D-ribofuranos-1-yl)purine in the post-reaction solution.

Provenance & Evidence