反应详情

A solution of 0.2 mg of bovine pancreatic insulin (Wako Pure Chemical Industries, Itd.) in 1 mL of a 2 mmol/L aqueous solution of hydrochloric acid, a solution of 320 mg of anhydrous β-lactose (Nacalai Tesque, Inc.) in 2 mL of distilled water, and 1 mL of a 200 mmol/L phosphate buffer (pH 8.4) were mixed in a test tube to give a mixture having a pH of about 8.1. The test tube was put into a constant temperature water bath at 40° C. to cause reaction. The reaction mixture was sampled after 0, 5 and 24 hours, and analyzed for products obtained by the reaction of insulin with lactose by high performance liquid chromatography (HPLC) under the following conditions.

1. customto give a mixture
2. customThe test tube was put into a constant temperature water bath at 40° C.
3. customreaction
4. aliquotThe reaction mixture was sampled after 0, 5 and 24 hours