Demannose → D-(-)-Fructose

HRID 1835040Notes

Reaction equation

HRID 1835040 reaction equation
[O:1]=[CH:2][C@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4]>P([O-])([O-])([O-])=O>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

3

Reactant 1

HCID 161658Demannose

Product 1

HCID 5984D-(-)-Fructose

Reagents, catalysts & solvents 1

HCID 1061PhosphateSolvent

Procedure

The activity of the MIase was measured by the following method. First, 200 μl of an enzyme solution that had been diluted as appropriate was added to 200 μl of 200 mM mannose solution dissolved in a 100 mM phosphate buffer (pH7.0), and an enzyme reaction was performed at 50° C. Then, the enzyme reaction was stopped by boiling for 10 minutes, and the composition of the saccharide was analyzed by HPLC. The amount of the enzymes that produce 1 μmol of fructose for 1 minute under these conditions was defined as one unit.

Workup

  1. customan enzyme reaction
  2. customwas performed at 50° C
  3. customThen, the enzyme reaction

Sources & evidence

ORD record
ord-549fa36efa314ae3a178a99a20b8d587
Source dataset
uspto-grants-2004_08
Patent number
US06770475B1

Reaction notes

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