Demannose → D-(-)-Fructose

Participants

Reaction Steps

The activity of the MIase was measured by the following method. First, 200 μl of an enzyme solution that had been diluted as appropriate was added to 200 μl of 200 mM mannose solution dissolved in a 100 mM phosphate buffer (pH7.0), and an enzyme reaction was performed at 50° C. Then, the enzyme reaction was stopped by boiling for 10 minutes, and the composition of the saccharide was analyzed by HPLC. The amount of the enzymes that produce 1 μmol of fructose for 1 minute under these conditions was defined as one unit.

Provenance & Evidence