Silver Nitrate → Silver Nitrate

Participants

Reaction Steps

The chlorhexidine/silver nitrate formulation was prepared by dissolving 22.5 mg of chlorhexidine (3% w/w based on the dried film weight) in 10 ml of a methyl acetate/acetone solvent mixture (70/30, vol/vol) at room temperature with gentle swirling. The chlorhexidine dissolved quickly to yield a clear solution. Next, 750 mg of the polymer of Example 1 (7.5% weight/volume) was added to the solution. The solution was agitated and shaken gently to facilitate dissolution of the polymer. Upon complete dissolution of the polymer after approximately 6 hours, 150 mg of silver nitrate crystals (excess) was added to the solution in a roller bottle. The roller bottle was wrapped with aluminum foil and placed on a rolling apparatus for sufficient time to permit dissolved silver nitrate to come to equilibrium with the remaining solid silver nitrate to produce a saturated silver nitrate solution. After approximately 16 hours, the solution was filtered through 0.45 μm Acrodisc filters to remove the undissolved silver nitrate and collected in a clean vial for future use. The vial containing the filtered solution was wrapped with aluminum foil to avoid contact with light and stored at 4° C.

Provenance & Evidence