Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Hydrochloric Acid | HCID 313 | — |
| Reactant | Thiamine | HCID 1130 | — |
| Reactant | Calcium Carbonate | HCID 10112 | — |
| Reactant | Potassium Hydroxide | HCID 14797 | — |
| Reactant | Sulfuric acid magnesium salt (1:1) | HCID 24083 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Biotin | HCID 171548 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Reactant | Ammonium Sulfate | HCID 6097028 | — |
| Catalyst | Manganese Sulfate | HCID 24580 | — |
| Product | 2-Isopropylmalic acid | HCID 77 | — |
Reaction Steps
An aqueous culture medium was prepared to contain 10 g/dl glucose, 1.5 g/dl ammonium sulfate, 0.1 g/dl KH2PO4, 0.04 g/dl MgSO4. 7H2O, 0.001 g/dl MnSO4. 4H2O, 250 μg/l biotin, 200 μg/l thiamine.HCl, 1.5 ml/dl soybean protein hydrolyzate (containing 6.5 g total nitrogen/dl) and 5 g/dl CaCO3 (separately sterilized) and adjusted to pH 7.0 with KOH. Twenty ml batches of the aqueous culture medium were placed in 500 ml shaking flasks and heated at 110° C. for 10 minutes with steam.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US04218261 |
|---|
| Source dataset | uspto-grants-1980_08 |
|---|
| ORD ID | ord-8c5ece5814624260ae895b841ea2cbf6 |
|---|
| Source note | uspto-grants-1980_08 |
|---|
