Hydrochloric Acid → 2-Isopropylmalic acid
Reaction equation
O=[CH:2][C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].OP([O-])(O)=O.[K+].[O-]S([O-])(=O)=O.[Mg+2].[OH:32][C:33]([CH2:35][CH2:36][CH2:37][CH2:38][C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:34].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[C:67]([O-:70])([O-])=[O:68].[Ca+2].[OH-:72].[K+]>[O-]S([O-])(=O)=O.[Mn+2]>[OH:72][C:36]([C:67]([OH:70])=[O:68])([CH:37]([CH3:38])[CH3:2])[CH2:35][C:33]([OH:32])=[O:34]Participants
11
Reactant 9
Product 1
Reagents, catalysts & solvents 1
Conditions
- Temperature
- 110 °C
Procedure
An aqueous culture medium was prepared to contain 10 g/dl glucose, 1.5 g/dl ammonium sulfate, 0.1 g/dl KH2PO4, 0.04 g/dl MgSO4. 7H2O, 0.001 g/dl MnSO4. 4H2O, 250 μg/l biotin, 200 μg/l thiamine.HCl, 1.5 ml/dl soybean protein hydrolyzate (containing 6.5 g total nitrogen/dl) and 5 g/dl CaCO3 (separately sterilized) and adjusted to pH 7.0 with KOH. Twenty ml batches of the aqueous culture medium were placed in 500 ml shaking flasks and heated at 110° C. for 10 minutes with steam.
Workup
- customAn aqueous culture medium was prepared
Sources & evidence
- ORD record
- ord-8c5ece5814624260ae895b841ea2cbf6
- Source dataset
- uspto-grants-1980_08
- Patent number
- US04218261
Reaction notes
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