反应详情

Fifty ml of the conversion culture medium described in Examples 1-9 was placed in a 250 ml Erlenmeyer flask and autoclaved at 120° C. for 20 minutes. This medium was inoculated with 1 ml of the seed culture solution prepared as in Examples 1-9 and shaking culture was carried out at 220 rpm for 48 hours at 28° C. To this culture were added cholesterol in the form of powder and 5.6 ml of a 1.5% aqueous solution of partially methylated β-cyclodextrin (methylation rate 74%: Mercian Corp.) which was sterilized beforehand, and the cultivation continued for further 40 hours. By the same method as Examples 1-9, 25-hydroxycholesterol in the culture solution obtained was quantified. The conversion rates into 25-hydroxycholesterol from cholesterol were summarized in Table 3.

1. customThis medium was inoculated with 1 ml of the seed culture solution prepared as in Examples 1-9
2. waitwas carried out at 220 rpm for 48 hours at 28° C
3. waitthe cultivation continued for further 40 hours