D-(-)-Fructose → Mannitol

Participants

Reaction Steps

Also, the present Invention is to provide an isolating method for novel strain, Candida magnoliae (KCCM-10252), comprising steps of i) sorting a single colony growing fast at 27˜33° C. by using a plate containing 10˜20 g/L of glucose, 18˜22 g/L of fructose, 15˜25 g/L of peptone, 7˜15 g/L of yeast extract and 12˜17 g/L of agar after diluting a natural sludge sample; ii) sorting the strain producing mannitol most after fermenting such sorted strains in the medium containing 30˜70 g/L of glucose, 50˜100 g/L of fructose, 5˜20 g/L of yeast extract, 1˜5 g/L of ammonium sulfate and 1˜5 g/L of dibasic potassium phosphate for 68˜76 hours, and iii) isolating the novel strain, Candida magnoliae (KCCM-10252) producing the mannitol.

Provenance & Evidence