D-(-)-Fructose → Mannitol

HRID 1859388Notes

Reaction equation

HRID 1859388 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].OCC([C@H]([C@@H]([C@@H](CO)O)O)O)=O>>[CH2:11]([OH:12])[C@H:9]([C@H:7]([C@@H:5]([C@@H:3]([CH2:2][OH:1])[OH:4])[OH:6])[OH:8])[OH:10]

Participants

3

Reactant 2

HCID 5984D-(-)-FructoseHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 6251Mannitol

Procedure

Also, the present Invention is to provide an isolating method for novel strain, Candida magnoliae (KCCM-10252), comprising steps of i) sorting a single colony growing fast at 27˜33° C. by using a plate containing 10˜20 g/L of glucose, 18˜22 g/L of fructose, 15˜25 g/L of peptone, 7˜15 g/L of yeast extract and 12˜17 g/L of agar after diluting a natural sludge sample; ii) sorting the strain producing mannitol most after fermenting such sorted strains in the medium containing 30˜70 g/L of glucose, 50˜100 g/L of fructose, 5˜20 g/L of yeast extract, 1˜5 g/L of ammonium sulfate and 1˜5 g/L of dibasic potassium phosphate for 68˜76 hours, and iii) isolating the novel strain, Candida magnoliae (KCCM-10252) producing the mannitol.

Workup

  1. customto provide an isolating method for novel strain, Candida magnoliae (KCCM-10252)
  2. customgrowing fast at 27˜33° C.
  3. extractionextract
  4. addition12˜17 g/L of agar after diluting a natural sludge sample

Sources & evidence

ORD record
ord-1997e5972fd0468782d03f63cbe08d57
Source dataset
uspto-grants-2003_03
Patent number
US06528290B1

Reaction notes

Loading…