Ethanol → HCID 178418489, compound

Temperature

30 °C

D-arabitol was dissolved in a 0.1M phosphate buffer (pH=6.0) in such amount that the final concentration thereof was 5%(w/v), whereby a solution was prepared in an amount of 320 ml. Using the solution as the base culture medium (reaction solution), the above microbial cells collected and washed were added thereto in an amount of about 10%(w/v) in terms of wet weight. In order to prevent pH decrease of the culture medium during the progress of culturing or incubation (reaction), calcium carbonate was further added in an amount of 2%(w/v). Culturing was conducted with shaking at 30° C. Also, as the carbon source, glucose was added in an amount of 1%(w/v) at the beginning of the culturing, and after 6 hours from the beginning of the culturing, ethanol was added in an amount of 5%(w/v). After 24 hours from the beginning of the culturing, xylitol had been formed in an amount of 4.9 g per one dl of the culture broth (98% yield based on the D-arabitol).

1. concentrationconcentration
2. customwas prepared in an amount of 320 ml
3. customas the base culture medium (reaction solution)
4. customthe above microbial cells collected
5. washwashed
6. additionwere added
7. customincubation (reaction), calcium carbonate
8. additionwas further added in an amount of 2%(w/v)

ORD record

ord-84a75fbaeae24ab28b1caf2835558c73

Source dataset

uspto-grants-2003_03

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06538133B1