Phosphoric Acid → An inositol

HRID 1871185Notes

Reaction equation

HRID 1871185 reaction equation
[OH-].[Na+].P(=O)(O)(O)O.[O:8]=[CH:9][C@@H:10]([C@H:12]([C@@H:14]([C@@H:16]([CH2:18][OH:19])[OH:17])[OH:15])[OH:13])[OH:11]>OCC(CO)O>[CH:18]1([OH:19])[CH:9]([OH:8])[CH:10]([OH:11])[CH:12]([OH:13])[CH:14]([OH:15])[CH:16]1[OH:17]

Participants

5

Reactant 3

HCID 1004Phosphoric AcidHCID 14798Sodium HydroxideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 892An inositol

Reagents, catalysts & solvents 1

HCID 753GlycerolSolvent

Conditions

Temperature
30 °C

Procedure

During growth of cells in the fermentation vessel, the medium was maintained at a temperature of 30° C. pH 6.0 was maintained by addition of NaOH or phosphoric acid as needed. Agitation was begun at 600 r.p.m. and increased as needed to maintain D.O >30%. Aeration was 1 vvm air. Starting at 15 h after inoculation, a mixture of sterile glucose (25% w/v) and glycerol (6.8% w/v) was added to the fermentor at a rate of 0.1 ml/min/L. At 39 hours, the rate of addition of this mixture was decreased to 0.05 ml/min/L. The culture was terminated at 96 hours. Peak expression of TFPI (1 mg/ml) occurred by 72 h post-inoculation.

Workup

  1. temperatureand increased
  2. temperatureto maintain D.O >30%
  3. additionwas added to the fermentor at a rate of 0.1 ml/min/L
  4. waitAt 39 hours
  5. additionthe rate of addition of this mixture
  6. customThe culture was terminated at 96 hours
  7. waitPeak expression of TFPI (1 mg/ml) occurred by 72 h

Sources & evidence

ORD record
ord-4b9d2211bbe14107bb82e4163cb39b4e
Source dataset
uspto-grants-2000_08
Patent number
US06103500

Reaction notes

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