HuagongsheHuagongshe Chemistry Print Report · For research reference only
Nadide → Nadh
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Nadide | HCID 5892 | — |
| Reactant | Sodium Hydroxide | HCID 14798 | — |
| Reactant | Sodium Azide | HCID 33557 | — |
| Reactant | N-(2-Acetamido)-2-aminoethanesulfonic acid | HCID 81832 | — |
| Reactant | Sodium N-(hydroxymethyl)glycinate | HCID 23664641 | — |
| Solvent | Water | HCID 962 | — |
| Product | Nadh | HCID 439153 | — |
Reaction Steps
One hundred ml of a second reagent composition were prepared as follows. Approximately 80 ml deionized water were dispensed into a container and 1.82 grams of N-[2-acetamido]-2-aminoethanesulfonic acid (ACES) were added and completely dissolved. The pH was adjusted to 6.0 with 2N sodium hydroxide (NaOH). Then 0.166 gram nicotinamide adenine dinucleotide (NAD) was added, followed by 0.095 gram sodium azide and 0.1 gram SUTTOCIDE A. The volume was adjusted to 100 ml with deionized water.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06107052 |
| Source dataset | uspto-grants-2000_08 |
| ORD ID | ord-7555cfff4abc4d81add98f4590f6dfe0 |
| Source note | uspto-grants-2000_08 |