Nadide → Nadh

HRID 1871848Notes

Reaction equation

HRID 1871848 reaction equation
C(NCC(N)=O)CS(O)(=O)=O.[OH-].[Na+].[CH:14]1[CH:19]=[N+:18]([C@@H:20]2[O:24][C@H:23]([CH2:25][O:26][P:27]([O:30][P:31]([O:34][CH2:35][C@H:36]3[O:40][C@@H:39]([N:41]4[C:45]5[N:46]=[CH:47][N:48]=[C:49]([NH2:50])[C:44]=5[N:43]=[CH:42]4)[C@H:38]([OH:51])[C@@H:37]3[OH:52])([OH:33])=[O:32])([O-:29])=[O:28])[C@@H:22]([OH:53])[C@H:21]2[OH:54])[CH:17]=[C:16]([C:55]([NH2:57])=[O:56])[CH:15]=1.[N-]=[N+]=[N-].[Na+].C(NCO)C([O-])=O.[Na+]>O>[CH:47]1[N:48]=[C:49]([NH2:50])[C:44]2[N:43]=[CH:42][N:41]([C@@H:39]3[O:40][C@H:36]([CH2:35][O:34][P:31]([O:30][P:27]([O:26][CH2:25][C@H:23]4[O:24][C@@H:20]([N:18]5[CH:17]=[C:16]([C:55]([NH2:57])=[O:56])[CH2:15][CH:14]=[CH:19]5)[C@H:21]([OH:54])[C@@H:22]4[OH:53])([OH:29])=[O:28])([OH:33])=[O:32])[C@@H:37]([OH:52])[C@H:38]3[OH:51])[C:45]=2[N:46]=1

Participants

7

Reactant 5

HCID 5892NadideHCID 14798Sodium HydroxideHCID 33557Sodium AzideHCID 81832N-(2-Acetamido)-2-aminoethanesulfonic acidHCID 23664641Sodium N-(hydroxymethyl)glycinate

Product 1

HCID 439153Nadh

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

One hundred ml of a second reagent composition were prepared as follows. Approximately 80 ml deionized water were dispensed into a container and 1.82 grams of N-[2-acetamido]-2-aminoethanesulfonic acid (ACES) were added and completely dissolved. The pH was adjusted to 6.0 with 2N sodium hydroxide (NaOH). Then 0.166 gram nicotinamide adenine dinucleotide (NAD) was added, followed by 0.095 gram sodium azide and 0.1 gram SUTTOCIDE A. The volume was adjusted to 100 ml with deionized water.

Workup

  1. additionwere added
  2. dissolutioncompletely dissolved

Sources & evidence

ORD record
ord-7555cfff4abc4d81add98f4590f6dfe0
Source dataset
uspto-grants-2000_08
Patent number
US06107052

Reaction notes

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