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FA was performed on the 14th day after intravitreal injection of Rapamycin. Briefly, a 25-gauge butterfly catheter was placed in the tail vein on an anesthetized animal and a Heparin-Lock solution (0.50 mL of 10,000 IU/mL Heparin Sodium with 0.95 mL 0.9% Saline) was used to fill the catheter line to maintain intravenous access. A dose of 10 mg/kg of Diofluor 10% (Fluorescein Sodium 10%) was delivered through the tail vein, followed by a flush of sterile saline to ensure full delivery of Diofluor 10%. An infusion pump (Becton-Dickinson) connected to a 60 cc syringe was used to infuse the Diofluor 10% at a constant rate of 6 mL/min (8.4-13.2 pounds per square inches) to allow for consistency in the synchronization of the fluorescein bolus injection and the angiogram acquisition. Photographs were taken with a fluorescence fundus camera at 1-10, 30, 60, 90, 180, and 300 sec after Diofluor 10% administration. The leakiness of CNV was assessed by two independent readers masked to the treatment.

1. temperatureto maintain intravenous access
2. customa flush of sterile saline
3. customAn infusion pump (Becton-Dickinson) connected to a 60 cc syringe
4. customwere taken with a fluorescence fundus camera at 1-10, 30, 60, 90, 180, and 300 sec