Fluorescein → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

HRID 1877721Notes

Reaction equation

HRID 1877721 reaction equation
C[C@H]1[C@@]2(O)O[C@H](C[C@H](OC)C(C)=CC=CC=C[C@@H](C)C[C@@H](C)C([C@H](OC)[C@H](O)C(C)=C[C@@H](C)C(C[C@@H]([C@@H](C[C@H]3C[C@@H](OC)[C@H](O)CC3)C)OC([C@H]3N(C(C2=O)=O)CCCC3)=O)=O)=O)CC1.[CH:66]1[CH:71]=[C:70]2[C:72]([O:74][C:75]3([C:85]4[CH:86]=[CH:87][C:88]([OH:90])=[CH:89][C:84]=4[O:83][C:77]4[CH:78]=[C:79]([OH:82])[CH:80]=[CH:81][C:76]3=4)[C:69]2=[CH:68][CH:67]=1)=[O:73]>>[CH:67]1[CH:66]=[CH:71][C:70]([C:72]([OH:74])=[O:73])=[C:69]([C:75]2[C:76]3[CH:81]=[CH:80][C:79]([OH:82])=[CH:78][C:77]=3[O:83][C:84]3[C:85]=2[CH:86]=[CH:87][C:88]([CH:89]=3)=[O:90])[CH:68]=1

Participants

3

Reactant 2

HCID 16850FluoresceinHCID 5284616Sirolimus

Product 1

HCID 33832-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

Procedure

FA was performed on the 14th day after intravitreal injection of Rapamycin. Briefly, a 25-gauge butterfly catheter was placed in the tail vein on an anesthetized animal and a Heparin-Lock solution (0.50 mL of 10,000 IU/mL Heparin Sodium with 0.95 mL 0.9% Saline) was used to fill the catheter line to maintain intravenous access. A dose of 10 mg/kg of Diofluor 10% (Fluorescein Sodium 10%) was delivered through the tail vein, followed by a flush of sterile saline to ensure full delivery of Diofluor 10%. An infusion pump (Becton-Dickinson) connected to a 60 cc syringe was used to infuse the Diofluor 10% at a constant rate of 6 mL/min (8.4-13.2 pounds per square inches) to allow for consistency in the synchronization of the fluorescein bolus injection and the angiogram acquisition. Photographs were taken with a fluorescence fundus camera at 1-10, 30, 60, 90, 180, and 300 sec after Diofluor 10% administration. The leakiness of CNV was assessed by two independent readers masked to the treatment.

Workup

  1. temperatureto maintain intravenous access
  2. customa flush of sterile saline
  3. customAn infusion pump (Becton-Dickinson) connected to a 60 cc syringe
  4. customwere taken with a fluorescence fundus camera at 1-10, 30, 60, 90, 180, and 300 sec

Sources & evidence

ORD record
ord-f9e8f4c823e54ea8a678b4aeb780eb14
Source dataset
uspto-grants-2012_11
Patent number
US08313763B2

Reaction notes

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