化工社化工社 化学情報印刷レポート · 研究参考用
NADP nicotinamide-adenine-dinucleotide phosphate → NADPH
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | NADP nicotinamide-adenine-dinucleotide phosphate | HCID 5886 | — |
| 反応物 | Glucose-6-Phosphate | HCID 439958 | — |
| 溶媒 | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| 生成物 | NADPH | HCID 5884 | — |
反応手順
A mixture of substrate (5 mM), oxidized cofactor NADP+ (10 μM), glucose 6-phosphate (20 mM) in 50 mM Tris buffer pH 7.5 (0.8 ml) was admixed with G6PDH (10 u), after the enzyme (100-200 μg) had been added, and the reaction was carried out at 30° C. (140 rpm) for 48 hours.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US08313923B2 |
| 出典データセット | uspto-grants-2012_11 |
| ORD ID | ord-df688da077ca401b833773d89a7294a5 |
| 出典メモ | uspto-grants-2012_11 |