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HRID1877742

反应详情

EQUATION

反应方程式

HRID 1877742 的结构方程式

REACTANTS

反应物

2
结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
HCID5886

Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium

C21H29N7O17P3+

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)COP(=O)(O)O
HCID439958

Glucose 6-phosphate

C6H13O9P

PRODUCTS

生成物

1
结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID5884

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：NC(CO)(CO)CO
溶剂HCID6503

Tromethamine

C4H11NO3

PROCEDURE

实验过程

A mixture of substrate (5 mM), oxidized cofactor NADP+ (10 μM), glucose 6-phosphate (20 mM) in 50 mM Tris buffer pH 7.5 (0.8 ml) was admixed with G6PDH (10 u), after the enzyme (100-200 μg) had been added, and the reaction was carried out at 30° C. (140 rpm) for 48 hours.

WORKUP

后处理

  1. additionafter the enzyme (100-200 μg) had been added
  2. customwas carried out at 30° C.
  3. customfor 48 hours