反应详情

FIG. 12 illustrates the synthesis of Mass Tag (3). To a mixture of N-Boc-β-tert-butyl-α-succinimido-aspartic acid (Boc-Asp(But)-OSu) (Bacchem, 0.1 mmol) in DMF (1 ml) was added hexamethyleneimine (Aldrich, 0.4 mmol). More DMF (2 ml) was added. The mixture was shaken at room temperature for 2 hours to form Boc-Asp(But)-HMI. Boc-Asp(But)-HMI was purified with preparative HPLC, and characterized with MS ([M+H]+: 371.3, calculated; 371.1, found).