Potassium Chloride → Nadh

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An experiment was conducted to measure, by chronoamperometry, the response of ferrocyanide generated bio-chemically from an enzyme catalysed electrochemical reaction between ferricyanide and nicotinamide adenine dinucleotide (NADH). Two solutions of ferricyanide solution comprising 25 mM K3[Fe(CN)6] (Aldrich) in Tris pH 9 buffer (Sigma) with 100 mM KCl (BDH), 50 mM MgSO4 (Sigma) and 1% sodium taurocholate (Sigma) were prepared. To one solution, Putidaredoxin reductase (PdR) was added and to the other diaphorase was added (both were used at a concentration of 5 mgml−1). NADH solutions were prepared at the following concentrations: 7, 5, 3, 2 and 1 mM in Tris pH 9 buffer (Sigma) with 100 mM KCl (BDH), 50 mM MgSO4 (Sigma) and 1% sodium taurocholate (Sigma).

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