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HRID1895814

反应详情

EQUATION

反应方程式

HRID 1895814 的结构方程式

REACTANTS

反应物

4
结构：[Cl-].[K+]
HCID4873

Potassium chloride

ClK

2 次

结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)([O-])OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
HCID5892

NAD

C21H27N7O14P2

结构：O=S(=O)([O-])[O-].[Mg+2]
HCID24083

Magnesium sulfate anhydrous

MgO4S

2 次

结构：C[C@H](CCC(=O)NCCS(=O)(=O)[O-])[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C.[Na+]
HCID23666345

未命名化合物

2 次

PRODUCTS

生成物

1
结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID439153

Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide

C21H29N7O14P2

AUXILIARIES

试剂、催化剂与溶剂

4
结构：[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.[Fe+3].[K+].[K+].[K+]
催化剂HCID26250

未命名化合物

结构：N#C[Fe-3](C#N)(C#N)(C#N)(C#N)C#N
催化剂HCID178511487

未命名化合物

2 次

结构：N#C[Fe-4](C#N)(C#N)(C#N)(C#N)C#N
催化剂HCID178511491

未命名化合物

结构：NC(CO)(CO)CO
溶剂HCID6503

Tromethamine

C4H11NO3

2 次

PROCEDURE

实验过程

An experiment was conducted to measure, by chronoamperometry, the response of ferrocyanide generated bio-chemically from an enzyme catalysed electrochemical reaction between ferricyanide and nicotinamide adenine dinucleotide (NADH). Two solutions of ferricyanide solution comprising 25 mM K3[Fe(CN)6] (Aldrich) in Tris pH 9 buffer (Sigma) with 100 mM KCl (BDH), 50 mM MgSO4 (Sigma) and 1% sodium taurocholate (Sigma) were prepared. To one solution, Putidaredoxin reductase (PdR) was added and to the other diaphorase was added (both were used at a concentration of 5 mgml−1). NADH solutions were prepared at the following concentrations: 7, 5, 3, 2 and 1 mM in Tris pH 9 buffer (Sigma) with 100 mM KCl (BDH), 50 mM MgSO4 (Sigma) and 1% sodium taurocholate (Sigma).

WORKUP

后处理

  1. customwere prepared
  2. additionTo one solution, Putidaredoxin reductase (PdR) was added and to the other diaphorase
  3. additionwas added (