Potassium Chloride → Nadh

HRID 1895814Notes

Reaction equation

HRID 1895814 reaction equation
[CH:1]1[CH:6]=[N+:5]([C@@H:7]2[O:11][C@H:10]([CH2:12][O:13][P:14]([O:17][P:18]([O:21][CH2:22][C@H:23]3[O:27][C@@H:26]([N:28]4[C:32]5[N:33]=[CH:34][N:35]=[C:36]([NH2:37])[C:31]=5[N:30]=[CH:29]4)[C@H:25]([OH:38])[C@@H:24]3[OH:39])([OH:20])=[O:19])([O-:16])=[O:15])[C@@H:9]([OH:40])[C@H:8]2[OH:41])[CH:4]=[C:3]([C:42]([NH2:44])=[O:43])[CH:2]=1.[Cl-].[K+].[O-]S([O-])(=O)=O.[Mg+2].C[C@@H]([C@@H]1[C@@]2(C)[C@@H](O)C[C@@H]3[C@@]4(C)CC[C@@H](O)C[C@H]4C[C@@H](O)[C@H]3[C@@H]2CC1)CCC(NCCS([O-])(=O)=O)=O.[Na+]>C(O)C(N)(CO)CO.[Fe-4](C#N)(C#N)(C#N)(C#N)(C#N)C#N.[Fe-3](C#N)(C#N)(C#N)(C#N)(C#N)C#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.[K+].[K+].[K+].[Fe+3]>[CH:34]1[N:35]=[C:36]([NH2:37])[C:31]2[N:30]=[CH:29][N:28]([C@@H:26]3[O:27][C@H:23]([CH2:22][O:21][P:18]([O:17][P:14]([O:13][CH2:12][C@H:10]4[O:11][C@@H:7]([N:5]5[CH:4]=[C:3]([C:42]([NH2:44])=[O:43])[CH2:2][CH:1]=[CH:6]5)[C@H:8]([OH:41])[C@@H:9]4[OH:40])([OH:16])=[O:15])([OH:20])=[O:19])[C@@H:24]([OH:39])[C@H:25]3[OH:38])[C:32]=2[N:33]=1

Participants

9

Reactant 4

HCID 4873Potassium Chloride2×HCID 5892NadideHCID 24083Sulfuric acid magnesium salt (1:1)2×HCID 23666345Sodium Taurocholate2×

Product 1

HCID 439153Nadh

Reagents, catalysts & solvents 4

HCID 26250Potassium ferricyanideCatalystHCID 178511487HCID 178511487, compoundCatalyst2×HCID 178511491HCID 178511491, compoundCatalystHCID 6503Tris(hydroxymethyl)aminomethaneSolvent2×

Procedure

An experiment was conducted to measure, by chronoamperometry, the response of ferrocyanide generated bio-chemically from an enzyme catalysed electrochemical reaction between ferricyanide and nicotinamide adenine dinucleotide (NADH). Two solutions of ferricyanide solution comprising 25 mM K3[Fe(CN)6] (Aldrich) in Tris pH 9 buffer (Sigma) with 100 mM KCl (BDH), 50 mM MgSO4 (Sigma) and 1% sodium taurocholate (Sigma) were prepared. To one solution, Putidaredoxin reductase (PdR) was added and to the other diaphorase was added (both were used at a concentration of 5 mgml−1). NADH solutions were prepared at the following concentrations: 7, 5, 3, 2 and 1 mM in Tris pH 9 buffer (Sigma) with 100 mM KCl (BDH), 50 mM MgSO4 (Sigma) and 1% sodium taurocholate (Sigma).

Workup

  1. customwere prepared
  2. additionTo one solution, Putidaredoxin reductase (PdR) was added and to the other diaphorase
  3. additionwas added (

Sources & evidence

ORD record
ord-c86473a76c1d4f5a94aa764b308f9782
Source dataset
uspto-grants-2013_08
Patent number
US08518236B2

Reaction notes

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