Biotin N-hydroxysuccinimide ester → Biotin

Participants

Reaction Steps

4.9 mg of biotin-NHS was dissolved in 0.25 mL DMSO, and added dropwise into 5 mL of 2.5% bovine serum albumin (BSA) in 100 mM sodium phosphate, pH 7.5. The solution was stirred for 2 h at room temperature. Unreacted biotin-NHS was removed by centrifugation using a 10K cutoff tube. BSA concentration was determined from its absorbance at 280 nm.

Provenance & Evidence