Biotin N-hydroxysuccinimide ester → Biotin

HRID 1910372Notes

Reaction equation

HRID 1910372 reaction equation
C1C(=O)N([O:8][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@@H:15]2[S:19][CH2:18][C@@H:17]3[NH:20][C:21]([NH:23][C@H:16]23)=[O:22])=[O:10])C(=O)C1>CS(C)=O.P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[OH:10][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@H:15]1[C@@H:16]2[C@@H:17]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:19]1)=[O:8]

Participants

4

Reactant 1

HCID 6710714Biotin N-hydroxysuccinimide ester2×

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 2

HCID 679Dimethyl SulfoxideSolventHCID 24243Trisodium PhosphateSolvent

Procedure

4.9 mg of biotin-NHS was dissolved in 0.25 mL DMSO, and added dropwise into 5 mL of 2.5% bovine serum albumin (BSA) in 100 mM sodium phosphate, pH 7.5. The solution was stirred for 2 h at room temperature. Unreacted biotin-NHS was removed by centrifugation using a 10K cutoff tube. BSA concentration was determined from its absorbance at 280 nm.

Workup

  1. customwas removed by centrifugation
  2. concentrationBSA concentration

Sources & evidence

ORD record
ord-636fbf58a7b44ba6bf79eec7c893a2fb
Source dataset
uspto-grants-2013_12
Patent number
US08603750B2

Reaction notes

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