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HRID1919266

反应详情

EQUATION

反应方程式

HRID 1919266 的结构方程式

REACTANTS

反应物

7
结构：Cc1ncc(COP(=O)(O)O)c(C=O)c1O
HCID1051

Pyridoxal phosphate

C8H10NO6P

结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
HCID5893

未命名化合物

结构：C[C@H](N)C(=O)O
HCID5950

L-​Alanine

C3H7NO2

结构：[Na+].[OH-]
HCID14798

Sodium Hydroxide

HNaO

结构：COc1ccc2c(c1)CC(=O)CC2
HCID77785

7-Methoxy-2-tetralone

C11H12O2

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
HCID107526

未命名化合物

结构：COc1ccc2c(c1)CC(N)CC2
HCID11608015

未命名化合物

2 次

PRODUCTS

生成物

1
结构：COc1ccc2c(c1)C[C@@H](N)CC2
HCID11008478

(2S)-7-Methoxy-1,2,3,4-tetrahydronaphthalen-2-amine

C11H15NO

PROCEDURE

实验过程

Into a flask containing 300 mg of a substrate 7-methoxy-2-tetralone, 460 mg of D-glucose, 3 mg of NAD+, 910 mg of L-alanine, and 4.0 mg of pyridoxal phosphate, a culture fluid of the recombinant E. coli HB101 (pNMTAPAG), obtained in Example 8, which expresses MTA, PALDH, and GDH was added so that total volume was 30 ml. The resultant product was stirred at 30° C. for 20 hours while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. The rate of conversion into 7-methoxy-2-aminotetraline after the reaction was 92.3%. The absolute configuration of 7-methoxy-2-aminotetraline was (S), and the optical purity was 97.0% e.e.

WORKUP

后处理

  1. custom(pNMTAPAG), obtained in Example 8, which
  2. additionwas added so that total volume was 30 ml