HuagongsheHuagongshe Chemistry Print Report · For research reference only
Choline → Amplex Red
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Choline | HCID 305 | — |
| Reactant | 4-Aminoantipyrine | HCID 2151 | — |
| Reactant | L-Alanine | HCID 5950 | — |
| Reactant | Choline Alfoscerate | HCID 657272 | — |
| Reactant | 1-Propanesulfonic acid, 3-(ethyl(3-methylphenyl)amino)-2-hydroxy-, monosodium salt | HCID 23676159 | — |
| Reactant | Sodium n-ethyl-n-(2-hydroxy-3-sulfopropyl)-3-methylaniline | HCID 45157628 | — |
| Reactant | HCID 178508930 | HCID 178508930 | — |
| Product | Amplex Red | HCID 167453 | — |
Reaction Steps
Lysophospholipase (EC 3.1.1.5) was obtained from Asachi Chemical Co. (Tokyo, Japan). Glycerophosphorylcholine phosphodiesterase (GPCP; EC 3.1.4.2), choline oxidase (COD; EC 1.1.3.17), peroxidase (EC 1.11.1.7), sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline dehydrate (TOOS) and 4-aminoantipyrine were purchased from Aldrich (Milwaukee, Wis.). 1-Palmitoyl-2-hydroxy-phosphatidylcholine was obtained from Avanti Polar-Lipids Inc. (Alabaster, Ala.). 10-acetyl-3,7-dihydroxyphenoxazine was obtained from Invitrogen (Carlsbad, Calif.).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08183050B2 |
| Source dataset | uspto-grants-2012_05 |
| ORD ID | ord-09ff5bb6cd0446f4a58a1735aa7ede1d |
| Source note | uspto-grants-2012_05 |