Choline → Amplex Red

HRID 1926598Notes

Reaction equation

HRID 1926598 reaction equation
C[N+](CCOP(O[CH2:12][C@H:13]([OH:16])[CH2:14]O)([O-])=O)(C)C.C1N([C@@H:22]2[O:26][C@H:25]([CH2:27]OP([O-])(CP([O-])(O)=O)=O)[C@@H:24](O)[C@H:23]2O)C2NC(N)=NC(=O)C=2N=1.[Na+].[Na+].[OH:47][CH2:48][CH2:49][N+](C)(C)C.[CH2:54](N(CC(O)CS(O)(=O)=O)C1C=CC=C(C)C=1)C.[Na].CCN(C1C=C(C)C=CC=1)CC(O)CS([O-])(=O)=O.[Na+].CC1N(C)N(C2C=CC=CC=2)C(=O)C=1N.[NH2:107][C@H:108]([C:110]([OH:112])=O)[CH3:109]>>[C:48]([N:107]1[C:108]2[CH:109]=[CH:12][C:13]([OH:16])=[CH:14][C:110]=2[O:112][C:23]2[C:22]1=[CH:54][CH:27]=[C:25]([OH:26])[CH:24]=2)(=[O:47])[CH3:49]

Participants

8

Reactant 7

HCID 305CholineHCID 21514-AminoantipyrineHCID 5950L-AlanineHCID 657272Choline AlfoscerateHCID 236761591-Propanesulfonic acid, 3-(ethyl(3-methylphenyl)amino)-2-hydroxy-, monosodium saltHCID 45157628Sodium n-ethyl-n-(2-hydroxy-3-sulfopropyl)-3-methylanilineHCID 178508930HCID 178508930, compound

Product 1

HCID 167453Amplex Red

Procedure

Lysophospholipase (EC 3.1.1.5) was obtained from Asachi Chemical Co. (Tokyo, Japan). Glycerophosphorylcholine phosphodiesterase (GPCP; EC 3.1.4.2), choline oxidase (COD; EC 1.1.3.17), peroxidase (EC 1.11.1.7), sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline dehydrate (TOOS) and 4-aminoantipyrine were purchased from Aldrich (Milwaukee, Wis.). 1-Palmitoyl-2-hydroxy-phosphatidylcholine was obtained from Avanti Polar-Lipids Inc. (Alabaster, Ala.). 10-acetyl-3,7-dihydroxyphenoxazine was obtained from Invitrogen (Carlsbad, Calif.).

Sources & evidence

ORD record
ord-09ff5bb6cd0446f4a58a1735aa7ede1d
Source dataset
uspto-grants-2012_05
Patent number
US08183050B2

Reaction notes

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