反应详情

To a cold (0° C.) solution of 874 mg of 1-(tert-butoxycarbonyl)-5-[N2, N3-bis(benzyloxycarbonyl)guanidino]-4-methylbenzimidazole in 2 mL of methylene chloride is added 2 mL of trifluoroacetic acid. The resulting solution is stirred at room temperature for 30 minutes, then rotary evaporated. The residue is purified by flash column chromatography on silica gel, eluting with 20% methanol/chloroform followed by recrystallization from hexanes/methylene chloride to provide 573 mg of 5-[N2,N3-bis(benzyloxycarbonyl)guanidino]-4-methylbenzimidazole as a white solid.

1. customThe residue is purified by flash column chromatography on silica gel
2. washeluting with 20% methanol/chloroform
3. customfollowed by recrystallization from hexanes/methylene chloride