HuagongsheHuagongshe Chemistry Print Report · For research reference only
L-Homoserine → L-Isoleucine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | L-Homoserine | HCID 12647 | — |
| Reactant | Sulfuric acid magnesium salt (1:1) | HCID 24083 | — |
| Reactant | Sodium Phosphate, Dibasic | HCID 24203 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Reactant | Calcium Chloride | HCID 5284359 | — |
| Product | L-Isoleucine | HCID 6306 | — |
Reaction Steps
selecting a microorganism belonging to Escherichia coli which forms colonies more than 0.6 mm in diameter when cultured in 3 to 7 days at 30° to 35° C. when grown on minimum agar medium containing 0.5 wt. % glucose, 0.02 wt. % L-homoserine, 0.3 wt. % potassium dihydrogen phosphate, 0.6 wt. % disodium hydrogen phosphate, 0.01 wt. % magnesium sulfate, 20 mg/liter calcium chloride, and 2 wt. % agar (pH 7.2) supplemented with 20 mg/liter auxotrophic material and produces L-isoleucine in a nutrient medium;
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05695972 |
| Source dataset | uspto-grants-1997_12 |
| ORD ID | ord-6256baf0adc64146990932cdda0ce3c6 |
| Source note | uspto-grants-1997_12 |