L-Homoserine → L-Isoleucine

HRID 1939135Notes

Reaction equation

HRID 1939135 reaction equation
O=[CH:2][C@@H:3]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[NH2:13][C@H:14]([C:18]([OH:20])=[O:19])[CH2:15][CH2:16]O.P([O-])(O)(O)=O.[K+].P([O-])([O-])(O)=O.[Na+].[Na+].S([O-])([O-])(=O)=O.[Mg+2].[Cl-].[Ca+2].[Cl-]>>[NH2:13][C@H:14]([C:18]([OH:20])=[O:19])[C@H:15]([CH2:2][CH3:3])[CH3:16]

Participants

7

Reactant 6

HCID 12647L-HomoserineHCID 24083Sulfuric acid magnesium salt (1:1)HCID 24203Sodium Phosphate, DibasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 5284359Calcium Chloride

Product 1

HCID 6306L-Isoleucine

Procedure

selecting a microorganism belonging to Escherichia coli which forms colonies more than 0.6 mm in diameter when cultured in 3 to 7 days at 30° to 35° C. when grown on minimum agar medium containing 0.5 wt. % glucose, 0.02 wt. % L-homoserine, 0.3 wt. % potassium dihydrogen phosphate, 0.6 wt. % disodium hydrogen phosphate, 0.01 wt. % magnesium sulfate, 20 mg/liter calcium chloride, and 2 wt. % agar (pH 7.2) supplemented with 20 mg/liter auxotrophic material and produces L-isoleucine in a nutrient medium;

Sources & evidence

ORD record
ord-6256baf0adc64146990932cdda0ce3c6
Source dataset
uspto-grants-1997_12
Patent number
US05695972

Reaction notes

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