Water → 2-Nitrophenol

HRID 1940467Notes

Reaction equation

HRID 1940467 reaction equation
[CH2:1]([OH:8])[C:2]([NH2:7])(CO)[CH2:3]O.Cl.S[CH2:11][CH:12]([CH:14](CS)O)O.S([O-])([O-])(=O)=[O:19].[Mg+2].[Cl-].[Li+].[OH2:26]>>[N+:7]([C:2]1[CH:3]=[CH:14][CH:12]=[CH:11][C:1]=1[OH:8])([O-:19])=[O:26]

Participants

6

Reactant 5

HCID 962WaterHCID 190011,4-Dimercapto-2,3-butanediolHCID 24083Sulfuric acid magnesium salt (1:1)HCID 93573Tromethamine hydrochlorideHCID 433294Lithium Chloride

Product 1

HCID 69472-Nitrophenol

Procedure

0.05 ml of the standard solution of sodium ion was placed in a test tube. Then, 2.0 ml of 300 mM Tris-HCl buffer (pH 7.4) preheated to 37° C. and containing 1100 unit/l β-galactosidase (Sigma), 3 mM DL-dithiothreitol (Sigma), 11.2 mM magnesium sulfate (Sigma) and 220 mM or 260 mM lithium chloride (Wako Pure Chemicals) was added to the test tube. Then, 1.0 ml of distilled water preheated to 37° C. and containing 1.5 mM o-nitrophenyl β-D-pyranoglycoside (Merck) was added thereto, mixed with agitation, and reacted at 37° C. The amount of o-nitrophenol produced for 1 minute was determined with a spectrophotometer (Hitachi; Model UV3400) based on the absorption intensity of the visible portion at 420 nm. The calibration curves obtained are shown in FIG. 1.

Workup

  1. custompreheated to 37° C.
  2. additionwas added to the test tube
  3. custompreheated to 37° C.
  4. additioncontaining 1.5 mM o-nitrophenyl β-D-pyranoglycoside (Merck)
  5. additionwas added
  6. additionmixed with agitation
  7. customreacted at 37° C

Sources & evidence

ORD record
ord-1d8dbc9955a74a41bfa6444566c82c96
Source dataset
uspto-grants-1997_12
Patent number
US05700652

Reaction notes

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