反应详情

To a solution of 5-[3-(2-ethyl-3-formylphenyl)-1,2,4-thiadiazol-5-yl]-2-(2-methylpropyl)benzonitrile (D63) (100 mg) in dichloromethane (DCM) (15 mL) was added sodium acetate (22.9 mg), ethyl 4-piperidinecarboxylate (46.1 mg) and 5 drops of acetic acid. After stirring for 30 min, sodium triacetoxyborohydride (85 mg) was added and the reaction solution was stirred at room temperature overnight. The solvent was removed in vacuo and the residue was dissolved in ethyl acetate (30 mL), the organic phase was washed with water, dried over sodium sulphate and concentrated. The residue was dissolved in isopropanol/water (10 mL, 1:1), 0.5M sodium hydroxide (2.66 mL) was added. The reaction solution was stirred at room temperature overnight. The isopropanol was removed in vacuo and the residue was acidified to pH=3-4. The resulting solution was extracted with ethyl acetate (2*15 mL), and the combined organic phases were dried over sodium sulphate, concentrated and purified by Mass Directed AutoPrep to afford 1-[(3-{5-[3-cyano-4-(2-methylpropyl)phenyl]-1,2,4-thiadiazol-3-yl}-2-ethylphenyl)methyl]-4-piperidinecarboxylic acid (E28) (43 mg). δH (DMSO-d6, 400 MHz): 0.87 (6H, d), 1.03 (3H, t), 1.47 (2H, m), 1.71 (2H, m), 1.92 (3H, m), 2.01 (1H, m), 2.70 (4H, m), 2.93 (2H, q), 3.47 (2H, s), 7.24 (1H, t), 7.39 (1H, dd), 7.66 (2H, m), 8.26 (1H, dd), 8.47 (1H, d).

1. stirringthe reaction solution was stirred at room temperature overnight
2. customThe solvent was removed in vacuo
3. dissolutionthe residue was dissolved in ethyl acetate (30 mL)
4. washthe organic phase was washed with water
5. dry with materialdried over sodium sulphate
6. concentrationconcentrated
7. dissolutionThe residue was dissolved in isopropanol/water (10 mL, 1:1)
8. addition0.5M sodium hydroxide (2.66 mL) was added
9. stirringThe reaction solution was stirred at room temperature overnight
10. customThe isopropanol was removed in vacuo
11. extractionThe resulting solution was extracted with ethyl acetate (2*15 mL)
12. dry with materialthe combined organic phases were dried over sodium sulphate
13. concentrationconcentrated
14. custompurified by Mass Directed AutoPrep