反应详情

An ethanol solution (2 mL) of 1,3-bis(methoxymethoxy)-4-{5-hydroxy-4-[4-(morpholin-4-yl)-phenyl]-4H-[1,2,4]triazol-3-yl}-6-isopropylbenzene (IM6-O-a01: 16.3 mg, 0.034 mmol) was mixed with 5 N hydrochloric acid (1 mL) and reacted at room temperature for 4 hours. The solvent was distilled off under reduced pressure, and the residue was neutralized with 5% sodium hydrogencarbonate and extracted with dichloromethane (30 mL). The extract was dried over anhydrous sodium sulfate. Sodium sulfate was removed by filtration, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (dichloromethane: methanol=30:1-20:1) to obtain the title compound (OH-a01: white solid, 3.0 mg, 22%).

1. customreacted at room temperature for 4 hours
2. distillationThe solvent was distilled off under reduced pressure
3. extractionextracted with dichloromethane (30 mL)
4. dry with materialThe extract was dried over anhydrous sodium sulfate
5. customSodium sulfate was removed by filtration
6. distillationthe solvent was distilled off under reduced pressure
7. customthe residue was purified by silica gel column chromatography (dichloromethane: methanol=30:1-20:1)