反应详情

100 mg (0.305 mmol) of the compound from example 14A and 25 mg (0.305 mmol) of 3-amino-crotononitrile are dissolved in 3 ml of isopropanol and stirred at the reflux temperature overnight. After cooling to room temperature, the volatile components are removed in a rotary evaporator, and the crude product is purified by preparative HPLC (eluent: acetonitrile/water with 0.1% formic acid, gradient 20:80→95:5). 59 mg (49% of theory) of the title compound are obtained.

1. customthe volatile components are removed in a rotary evaporator
2. customthe crude product is purified by preparative HPLC (eluent: acetonitrile/water with 0.1% formic acid, gradient 20:80→95:5)