(6-Aminohexanoyl)-4-amino-methylsalicylhydroxamic acid → Salicylhydroxamic Acid

HRID 1955361Notes

Reaction equation

HRID 1955361 reaction equation
NCCCCCC([C:9]1[C:18](N)=[CH:17][CH:16]=[C:11]([C:12]([NH:14][OH:15])=[O:13])[C:10]=1[O:20]C)=O>C([O-])(O)=O.[Na+]>[C:12]([NH:14][OH:15])(=[O:13])[C:11]1[C:10](=[CH:9][CH:18]=[CH:17][CH:16]=1)[OH:20]

Participants

3

Reactant 1

HCID 88168864(6-Aminohexanoyl)-4-amino-methylsalicylhydroxamic acid

Product 1

HCID 66644Salicylhydroxamic Acid

Reagents, catalysts & solvents 1

HCID 516892Sodium BicarbonateSolvent

Procedure

SHA-Sepharose 4B was prepared by mixing 130 mg of (6-aminohexanoyl)-4-amino-methylsalicylhydroxamic acid (SHA-Z-NH2), dissolved in 30 mL 0.2 M NaHCO3, with. 6.5 g HCl washed CNBr activated Sepharose 4B (Pharmacia) overnight at room temperature. After the coupling reaction, 2 mL 0.5 M Tris, pH 8.5 were added and the gel slurry mixed at room temperature for 1 hour, and washed with water, 0.5 M NaCl, and water again. The resulting SHA-Sepharose 4B was suspended in 30 mL of 20% ethanol, and stored at 4° C.

Workup

  1. wash6.5 g HCl washed CNBr
  2. customSepharose 4B (Pharmacia) overnight
  3. customat room temperature
  4. customAfter the coupling reaction, 2 mL 0.5 M Tris, pH 8.5
  5. additionwere added
  6. additionthe gel slurry mixed at room temperature for 1 hour
  7. washwashed with water, 0.5 M NaCl, and water again
  8. customstored at 4° C.

Sources & evidence

ORD record
ord-a5ef2c8e0332419ca75b320ba76d137a
Source dataset
uspto-grants-2000_09
Patent number
US06124471

Reaction notes

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