HRID1959

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@@H]2COP(=O)(O)O[C@H]2[C@H]1O

HCID6076

Cyclic AMP

C10H12N5O6P

结构：Nc1nc(=O)c2ncn([C@@H]3O[C@@H]4COP(=O)(O)O[C@H]4[C@H]3O)c2[nH]1

HCID178418274

未命名化合物

PRODUCTS

生成物

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)O)[C@@H](O)[C@H]1O

HCID6083

Adenosine 5'-phosphate

C10H14N5O7P

结构：Nc1nc(=O)c2ncn([C@@H]3O[C@H](COP(=O)(O)O)[C@@H](O)[C@H]3O)c2[nH]1

HCID178418270

未命名化合物

PROCEDURE

实验过程

The enzyme of the present invention isolated and purified from rat cerebral cortex in Example was acted on [8-3H] cAMP and [8-3H] cGMP as substrates to form [8-3H] 5'-AMP and [8-3H] 5'-GMP, respectively. The products were reacted with 5'-nucleotidase to form [8-3H] adenosine and [8-3H] guanosine, respectively. These products were separated from each other on a cation exchange resin column (AG50 W-X4, Bio-Rad) to assay for the radioactivity. The assay was carried out in a manner similar to that of the enzymatic activity described in Example.

WORKUP

后处理

customThe enzyme of the present invention isolated

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-4e4527ef977240138a93b4260c881157
来源数据集: uspto-grants-1998_03
DOI: 10.6084/m9.figshare.5104873.v1
专利: US05728563
来源说明: uspto-grants-1998_03

反应 SMILES

[CH:1]1[N:2]=[C:3]([NH2:22])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@@H:13]4[CH2:15][O:16][P:17]([OH:20])([O:19][C@H:12]4[C@H:11]3[OH:21])=[O:18])[C:5]=2[N:6]=1.[CH:23]1[N:27]([C@@H:28]2[O:32][C@@H:31]3[CH2:33][O:34][P:35]([OH:38])([O:37][C@H:30]3[C@H:29]2[OH:39])=[O:36])[C:26]2[NH:40][C:41]([NH2:45])=[N:42][C:43](=[O:44])[C:25]=2[N:24]=1>>[CH:1]1[N:2]=[C:3]([NH2:22])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([OH:20])([OH:19])=[O:18])[C@@H:12]([OH:32])[C@H:11]3[OH:21])[C:5]=2[N:6]=1.[CH:23]1[N:27]([C@@H:28]2[O:32][C@H:31]([CH2:33][O:34][P:35]([OH:38])([OH:37])=[O:36])[C@@H:30]([OH:14])[C@H:29]2[OH:39])[C:26]2[NH:40][C:41]([NH2:45])=[N:42][C:43](=[O:44])[C:25]=2[N:24]=1