HuagongsheHuagongshe Chemistry Print Report · For research reference only
Camp → Adenosine Monophosphate
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Camp | HCID 6076 | — |
| Reactant | HCID 178418274 | HCID 178418274 | — |
| Product | Adenosine Monophosphate | HCID 6083 | — |
| Product | HCID 178418270 | HCID 178418270 | — |
Reaction Steps
The enzyme of the present invention isolated and purified from rat cerebral cortex in Example was acted on [8-3H] cAMP and [8-3H] cGMP as substrates to form [8-3H] 5'-AMP and [8-3H] 5'-GMP, respectively. The products were reacted with 5'-nucleotidase to form [8-3H] adenosine and [8-3H] guanosine, respectively. These products were separated from each other on a cation exchange resin column (AG50 W-X4, Bio-Rad) to assay for the radioactivity. The assay was carried out in a manner similar to that of the enzymatic activity described in Example.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05728563 |
| Source dataset | uspto-grants-1998_03 |
| ORD ID | ord-4e4527ef977240138a93b4260c881157 |
| Source note | uspto-grants-1998_03 |