反应详情

A 20-mL vial was charged with (3S)-3-methyl-4-(((2S)-4-(2-thiophenylsulfonyl)-2-piperazinyl)methyl)morpholine (0.750 g, 2.17 mmol, Example 82), 2-(4-bromophenyl)-3,3,3-trifluoro-1,2-propanediol (0.766 g, 2.69 mmol, Example 67), 6 mL of toluene and sodium tert-butoxide (1.426 mL, 11.65 mmol). After stirring at room temperature for 5 min, dicyclohexyl(2′,6′-diisopropoxybiphenyl-2-yl)phosphine (RuPhos) (0.167 g, 0.359 mmol, Strem Chemicals Inc, Newburyport, Mass.) and tris(dibenzylideneacetone)dipalladium (0) (0.164 g, 0.179 mmol, Strem Chemicals Inc, Newburyport, Mass.) were added. The vial was sealed and heated at 100° C. for 1 h. After that time the mixture was cooled to room temperature, diluted with 50 mL MeOH, and filtered. The filtrate was concentrated, re-dissolved in 10 mL of CH2Cl2 and purified via column chromatography on silica gel (80 g, 0 to 75% EtOAc in hexanes) to give 3,3,3-trifluoro-2-(4-((2S)-2-(((3S)-3-methyl-4-morpholinyl)methyl)-4-(2-thiophenylsulfonyl)-1-piperazinyl)phenyl)-1,2-propanediol as a mixture of two isomers (0.109 g). 1H NMR (400 MHz, CD3OD) δ=7.91-7.80 (m, 1H), 7.64 (dd, J=1.2, 3.7 Hz, 1H), 7.45 (d, J=8.8 Hz, 2H), 7.26 (dd, J=3.7, 4.9 Hz, 1H), 6.93 (d, J=9.0 Hz, 2H), 4.09-3.98 (m, 3H), 3.93-3.85 (m, 1H), 3.79-3.37 (m, 6H), 3.26-3.10 (m, 2H), 2.89-2.81 (m, 1H), 2.64-2.45 (m, 2H), 2.37-2.25 (m, 1H), 2.14-1.99 (m, 1H), 1.94-1.81 (m, 1H), 1.00 (d, J=6.3 Hz, 3H). m/z (ESI, +ve ion) 550.1 (M+H)+. GK-GKRP EC50 (LC MS/MS-2)=0.019 μM.

1. customThe vial was sealed
2. temperatureheated at 100° C. for 1 h
3. temperatureAfter that time the mixture was cooled to room temperature
4. filtrationfiltered
5. concentrationThe filtrate was concentrated
6. dissolutionre-dissolved in 10 mL of CH2Cl2
7. custompurified via column chromatography on silica gel (80 g, 0 to 75% EtOAc in hexanes)