Sodium Carbonate → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

HRID 1973234Notes

Reaction equation

HRID 1973234 reaction equation
C([O-])([O-])=O.[Na+].[Na+].[CH:7]1[C:12](N=C=S)=[CH:11][C:10]2[C:16]([O:18][C:19]3([C:29]4[CH:30]=[CH:31][C:32]([OH:34])=[CH:33][C:28]=4[O:27][C:21]4[CH:22]=[C:23]([OH:26])[CH:24]=[CH:25][C:20]3=4)[C:9]=2[CH:8]=1)=[O:17]>P([O-])([O-])([O-])=O.CC(C)=O>[CH:7]1[CH:12]=[CH:11][C:10]([C:16]([OH:18])=[O:17])=[C:9]([C:19]2[C:20]3[CH:25]=[CH:24][C:23]([OH:26])=[CH:22][C:21]=3[O:27][C:28]3[C:29]=2[CH:30]=[CH:31][C:32]([CH:33]=3)=[O:34])[CH:8]=1

Participants

5

Reactant 2

HCID 10340Sodium CarbonateHCID 18730Fluorescein 5-isothiocyanate

Product 1

HCID 33832-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

Reagents, catalysts & solvents 2

HCID 180AcetoneSolventHCID 1061PhosphateSolvent

Procedure

One mg of HIgG (Human IgG) dissolved in 0.4ml of 0.1M phosphate buffer pH 7.5, was brought to pH 9.5 with crystalline Na2CO3. A solution (10μ1) of FITC (70μg) in acetone was added with stirring and mixed for 3 hrs at room temperature. The resulting solution was separated two times on Sephadex G-25(M) column (1×15cm) equilibrated with 0.05M phosphate buffer pH 8.0. The eluted FITC-hIgG conjugate solution was 0.58mg/ml in concentration and had D/P = 5.5 (M/M) as determined by the Wells' nomograph.

Workup

  1. additionmixed for 3 hrs at room temperature
  2. customThe resulting solution was separated two times on Sephadex G-25(M) column (1×15cm)
  3. concentrationin concentration

Sources & evidence

ORD record
ord-371f1b91159a4c48922c0a3214dc8d75
Source dataset
uspto-grants-1976_12
Patent number
US03996345

Reaction notes

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