Sodium Carbonate → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

Participants

Reaction Steps

One mg of HIgG (Human IgG) dissolved in 0.4ml of 0.1M phosphate buffer pH 7.5, was brought to pH 9.5 with crystalline Na2CO3. A solution (10μ1) of FITC (70μg) in acetone was added with stirring and mixed for 3 hrs at room temperature. The resulting solution was separated two times on Sephadex G-25(M) column (1×15cm) equilibrated with 0.05M phosphate buffer pH 8.0. The eluted FITC-hIgG conjugate solution was 0.58mg/ml in concentration and had D/P = 5.5 (M/M) as determined by the Wells' nomograph.

Provenance & Evidence