Glycine → L-Ascorbic Acid

Participants

Reaction Steps

In a similar airlift tower experiment a high cell density fermentation was performed. In this instance a 24 hour wet cell paste of C. norvegensis KCC MF-42 was dispersed in the 4.0 liter tower at a level of 100 GL-1 in SM-1 medium containing 0.7% glycine and 0.7% L-Galactono-gamma-lactone. Ethanol was supplied continuously at a level (0.1-0.3%) neither limiting or inhibitory to yeast growth or productivity. Oxygen-enriched aeration was supplied to the fermentor at 1:1 oxygen-air ratio. Total mixed gas volume was 1.7 V/V/min. Under these conditions a dissolved oxygen saturation level of 30% was maintained in the upper section of the tower. After 20 hours of fermentation, L-ascorbic acid was produced at a level of 1.44 GL-1.

Provenance & Evidence