Glycine → L-Ascorbic Acid

HRID 1982151Notes

Reaction equation

HRID 1982151 reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH:16]([OH:65])[CH:17]([NH:46][C:47]([CH:49]([OH:64])[CH2:50][CH2:51][CH2:52][CH2:53][CH2:54][CH2:55][CH2:56][CH2:57][CH2:58][CH2:59][CH2:60][CH2:61][CH2:62][CH3:63])=[O:48])[CH2:18][O:19][P:20]([O:23][C@H:24]1[C@@H:29]([O:30][C@H:31]2[O:36][C@H:35]([CH2:37][OH:38])[C@@H:34]([OH:39])[C@H:33]([OH:40])[C@H:32]2[NH2:41])[C@@H:28]([OH:42])[C@H:27]([OH:43])[C@@H:26]([OH:44])[C@@H:25]1[OH:45])([OH:22])=[O:21].NCC(O)=O.C(O)[C@H](O)[C@H]1OC(=O)[C@@H](O)[C@@H]1O.O=O>C(O)C>[O:36]=[C:31]1[O:30][C@H:29]([C@H:24]([CH2:25][OH:45])[OH:23])[C:28]([OH:42])=[C:27]1[OH:43].[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH:16]([OH:65])[CH:17]([NH:46][C:47]([CH:49]([OH:64])[CH2:50][CH2:51][CH2:52][CH2:53][CH2:54][CH2:55][CH2:56][CH2:57][CH2:58][CH2:59][CH2:60][CH2:61][CH2:62][CH3:63])=[O:48])[CH2:18][O:19][P:20]([O:23][C@H:24]1[C@@H:29]([O:30][C@H:31]2[O:36][C@H:35]([CH2:37][OH:38])[C@@H:34]([OH:39])[C@H:33]([OH:40])[C@H:32]2[NH2:41])[C@@H:28]([OH:42])[C@H:27]([OH:43])[C@@H:26]([OH:44])[C@@H:25]1[OH:45])([OH:22])=[O:21]

Participants

7

Reactant 4

HCID 750GlycineHCID 977Oxygen3×HCID 6857365L-Galactono-1,4-lactoneHCID 101244018HCID 101244018, compound

Product 2

HCID 54670067L-Ascorbic AcidHCID 101244018HCID 101244018, compound

Reagents, catalysts & solvents 1

HCID 702EthanolSolvent

Procedure

In a similar airlift tower experiment a high cell density fermentation was performed. In this instance a 24 hour wet cell paste of C. norvegensis KCC MF-42 was dispersed in the 4.0 liter tower at a level of 100 GL-1 in SM-1 medium containing 0.7% glycine and 0.7% L-Galactono-gamma-lactone. Ethanol was supplied continuously at a level (0.1-0.3%) neither limiting or inhibitory to yeast growth or productivity. Oxygen-enriched aeration was supplied to the fermentor at 1:1 oxygen-air ratio. Total mixed gas volume was 1.7 V/V/min. Under these conditions a dissolved oxygen saturation level of 30% was maintained in the upper section of the tower. After 20 hours of fermentation, L-ascorbic acid was produced at a level of 1.44 GL-1.

Workup

  1. customa 24 hour wet cell paste of C
  2. additionTotal mixed gas volume

Sources & evidence

ORD record
ord-5d85b896f3714626b6259bddc29c00b3
Source dataset
uspto-grants-1986_06
Patent number
US04595659

Reaction notes

Loading…