Sodium Carbonate → 9,12,15-Octadecatrienoic acid, sodium salt, (Z,Z,Z)-

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α-Linolenic acid serving as a starting material has considerably low water solubility. Therefore, in order to cause α-linolenic acid to function effectively as an enzyme substrate, an α-linolenic acid sodium salt was prepared. Specifically, sodium carbonate (530 mg) was dissolved in purified water (10 mL), and then heated to 55° C. α-Linolenic acid (product of Nacalai Tesque, Inc.) (278 mg) was added dropwise to the resultant solution, and the mixture was stirred for three hours.

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