(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → L-Lysine

HRID 1987692Notes

Reaction equation

O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)O)O)O.S([O-])([O-])(=O)=[O:14].[NH4+:18].[NH4+:19]>>[NH2:18][C@H:9]([C:11]([OH:12])=[O:14])[CH2:7][CH2:5][CH2:3][CH2:2][NH2:19]

Participants

Reactant 2

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HCID 6097028Ammonium Sulfate

Product 1

HCID 5962L-Lysine

Conditions

Temperature: 31.5 °C

Procedure

Brevibacterium flavum AJ 11275 (NRRL B-11474) was inoculated into a medium (pH 7.2) containing glucose 100 g/L, ammonium sulfate 8.0 g/L, Yeast Extract (Basco) 1.05 g/L, KH2PO4 1.0 g/L, MgSO4.7H2O 0.4 g/L, FeSO4.7H2O 10 mg/L, MnSO4.4H2O 10 mg/L, vitamin B, hydrochloride 0.2 mg/L, biotin 0.05 mg/L, and Surfactant GD-113 (Nippon Jushi) 0.05 mg/L, and culture with stirring at 31.5° C. for 70 hours to obtain L-lysine fermentation broth. The L-lysine content of the obtained broth was 3.46 wt %.

Workup

extractionExtract (Basco) 1.05 g/L, KH2PO4 1.0 g/L, MgSO4.7H2O 0.4 g/L, FeSO4.7H2O 10 mg/L, MnSO4.4H2O 10 mg/L, vitamin B, hydrochloride 0.2 mg/L, biotin 0.05 mg/L, and Surfactant GD-113 (Nippon Jushi) 0.05 mg/L, and culture

Sources & evidence

ORD record: ord-e9a91625209b4da3a2d628c7b1dff575
Source dataset: uspto-grants-2009_06
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07553367B2

Reaction notes

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