反应详情

温度

80 °C

64 mg of 6-(4-chlorobenzyl)-1-(2,4-dichlorobenzenesulfonyl)-3-amino-8-isopropylhexahydropyrazino[1,2-a]pyrimidine-4,7-dione hydrobromide (Example 2) were suspended in 1 ml of water, and 1 ml of DCE was added. This mixture was mixed with 2 equivalents of 2,5-dimethoxytetrahydrofuran and stirred at 80° C. for 1 hour. The DCE phase was removed and the aqueous phase was extracted twice with DCE. The combined extracts were evaporated, and the crude substance was dissolved in a mixture of acetonitrile and water and freeze dried. The pure title compound was removed after purification by HPLC. The system and process described under “general processes” was used for this. MW=608.08 (calculated, monoisotopic); measured value (M+H)+: 609.4.

1. customThe DCE phase was removed
2. extractionthe aqueous phase was extracted twice with DCE
3. customThe combined extracts were evaporated
4. dissolutionthe crude substance was dissolved in a mixture of acetonitrile and water and freeze
5. customdried
6. customThe pure title compound was removed
7. customafter purification by HPLC