反应详情

3.4 mg of 4-benzylamino-6-[4-methoxybenzyl-(6-morpholinopyridin-3-yl)amino]pyridine-3-carboxylic acid was dissolved in 1 mL of dichloromethane, to which 1.9 mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and 1.5 mg of 1-hydroxybenzotriazole monohydrate were added, and stirred at room temperature for 30 minutes. Then, 1 mL of 28% ammonia water was added, and stirred at the same temperature for 3 hours. To the reaction mixture water was added, extracted with chloroform, the extract was washed with saturated saline, and dried on anhydrous sodium sulfate. The solvent was evaporated to obtain 3.0 mg of the title compound containing impurities as a brown solid. Without further purification, this was used in the next reaction as it was.

1. additionwere added
2. stirringstirred at the same temperature for 3 hours
3. extractionextracted with chloroform
4. washthe extract was washed with saturated saline
5. dry with materialdried on anhydrous sodium sulfate
6. customThe solvent was evaporated