Sodium Chloride → Folic Acid

Participants

Reaction Steps

The strain KY 7985, NRRL 11189 is obtained in the following manner. Microbial cells of the parent strain, i.e. Norcardia butanica ATCC 21197, are suspended at a concentration of about 108 cells per ml, in 0.1 M-Tris-maleate buffer solution (pH 6.0). To the suspension is added 0.5 mg/ml of N-methyl-N'-nitro-N-nitrosoguanidine and the mixture is allowed to stand at room temperature for 30 minutes. Then the resultant suspension is smeared on an agar plate of a nutrient medium comprising 0.5 g/dl glucose, 0.5 g/dl yeast extract, 1 g/dl peptone, 1 g/dl meat extract, 0.5 g/dl NaCl and 2 g/dl agar (pH 7.2) and incubated at 30° C. for 2 days to form colonies. Cells of the resulting colonies are smeared on an agar plate of a minimum medium comprising 0.5 g/dl glucose, 0.2 g/dl (NH4)2SO4, 0.15 g/dl KH2PO4, 0.05 g/dl K2HPO4, 0.05 g/dl MgSO4 ·7H2O, 0.01 g/dl NaCl, 0.001 g/dl FeSO4 ·7H2O, 0.001 g/dl MnSO4 ·nH2O, 0.001 g/dl CaCl2 ·2H2O, 100 μg/l biotin, 1 mg/l thiamine hydrochloride, and 2 g/dl agar (pH 7.2) and also on an agar plate of a medium having the same composition as the minimum medium described above except using 0.2 g/dl L-serine instead of (NH4)2SO4.

Provenance & Evidence