Sodium Chloride → Folic Acid

HRID 2052239Notes

Reaction equation

HRID 2052239 reaction equation
C(O)[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3]O.C(O)[C:10]([NH2:15])([CH2:13]O)[CH2:11][OH:12].[CH:17](/[C:22]([OH:24])=O)=[CH:18]/[C:19](O)=O.C[N:26](N=O)[C:27]([NH:29][N+]([O-])=O)=[NH:28].[O:35]=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.OP([O-])(O)=O.[K+].OP([O-])([O-])=O.[K+].[K+].[O-]S([O-])(=O)=O.[Mg+2].[Na+].[Cl-].[Cl-].[Cl-].[Ca+2].[OH:71][C:72]([CH2:74]CCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:73].C[C:88]1[N+:92]([CH2:93][C:94]2[CH:95]=[N:96]C(C)=NC=2N)=CS[C:89]=1[CH2:102]CO.Cl.[Cl-].N[C@H](C(O)=O)CO>[O-]S([O-])(=O)=O.[Mn+2]>[C:72]([OH:73])(=[O:71])[CH2:74][CH2:13][C@H:10]([NH:15][C:22]([C:17]1[CH:18]=[CH:19][C:88]([NH:92][CH2:93][C:94]2[N:7]=[C:2]3[C:3]([N:28]=[C:27]([NH:29][C:5]3=[O:6])[NH2:26])=[N:96][CH:95]=2)=[CH:89][CH:102]=1)=[O:24])[C:11]([OH:12])=[O:35]

Participants

13

Reactant 11

HCID 5234Sodium ChlorideHCID 5951L-SerineHCID 6202Thiamine HydrochlorideHCID 24083Sulfuric acid magnesium salt (1:1)HCID 24450Potassium Phosphate, DibasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×HCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 5284359Calcium ChlorideHCID 645041872200-76-1HCID 135436526N-Methyl-N'-nitro-N-nitrosoguanidine

Product 1

HCID 135398658Folic Acid

Reagents, catalysts & solvents 1

HCID 24580Manganese SulfateCatalyst

Procedure

The strain KY 7985, NRRL 11189 is obtained in the following manner. Microbial cells of the parent strain, i.e. Norcardia butanica ATCC 21197, are suspended at a concentration of about 108 cells per ml, in 0.1 M-Tris-maleate buffer solution (pH 6.0). To the suspension is added 0.5 mg/ml of N-methyl-N'-nitro-N-nitrosoguanidine and the mixture is allowed to stand at room temperature for 30 minutes. Then the resultant suspension is smeared on an agar plate of a nutrient medium comprising 0.5 g/dl glucose, 0.5 g/dl yeast extract, 1 g/dl peptone, 1 g/dl meat extract, 0.5 g/dl NaCl and 2 g/dl agar (pH 7.2) and incubated at 30° C. for 2 days to form colonies. Cells of the resulting colonies are smeared on an agar plate of a minimum medium comprising 0.5 g/dl glucose, 0.2 g/dl (NH4)2SO4, 0.15 g/dl KH2PO4, 0.05 g/dl K2HPO4, 0.05 g/dl MgSO4 ·7H2O, 0.01 g/dl NaCl, 0.001 g/dl FeSO4 ·7H2O, 0.001 g/dl MnSO4 ·nH2O, 0.001 g/dl CaCl2 ·2H2O, 100 μg/l biotin, 1 mg/l thiamine hydrochloride, and 2 g/dl agar (pH 7.2) and also on an agar plate of a medium having the same composition as the minimum medium described above except using 0.2 g/dl L-serine instead of (NH4)2SO4.

Workup

  1. customThe strain KY 7985, NRRL 11189 is obtained in the following manner
  2. extractionextract
  3. extraction1 g/dl peptone, 1 g/dl meat extract
  4. wait0.5 g/dl NaCl and 2 g/dl agar (pH 7.2) and incubated at 30° C. for 2 days
  5. customto form colonies

Sources & evidence

ORD record
ord-00368753a8f1466b8f2cc68d64ce9fb5
Source dataset
uspto-grants-1980_01
Patent number
US04183786

Reaction notes

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