NADP nicotinamide-adenine-dinucleotide phosphate → 2-Chloro-1-(3-chloro-phenyl)-ethanol

Participants

Reaction Conditions

Reaction Steps

The culture of the recombinant Escherichia coli HB101(pNTRG) as obtained in Example 8 was subjected to ultrasonic cell disruption using SONIFIRE 250 (product of BRANSON). To 20 ml of this cell disruption fluid, there were added 2,000 U of glucose dehydrogenase (product of Amano Pharmaceutical Co., Ltd.), 3 g of glucose, 2 mg of NADP and 2 g of 2-chloro-1-(3′-chlorophenyl)ethanone. This reaction mixture was stirred at 30° C. for 18 hours while adjusting the pH to 6.5 by addition of 5 M sodium hydroxide. After completion of the reaction, this reaction mixture was extracted with toluene, the solvent was then removed, and the extract was analyzed. 2-Chloro-1-(3′-chlorophenyl)ethanol was obtained in 96% yield. The 2-chloro-1-(3′-chlorophenyl)ethanol formed on that occasion was the R form with an optical purity of 99.9% ee.

Provenance & Evidence