HRID2055066

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1

HCID5886

Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium

C21H29N7O17P3+

HCID14798

Sodium Hydroxide

HNaO

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO

HCID107526

未命名化合物

2 次

HCID168006

Ethanone, 2-chloro-1-(3-chlorophenyl)-

C8H6Cl2O

PRODUCTS

生成物

HCID11469563

2-Chloro-1-(3-chloro-phenyl)-ethanol

C8H8Cl2O

收率 96%

CONDITIONS

反应条件

温度: 30 °C

PROCEDURE

实验过程

The culture of the recombinant Escherichia coli HB101(pNTRG) as obtained in Example 8 was subjected to ultrasonic cell disruption using SONIFIRE 250 (product of BRANSON). To 20 ml of this cell disruption fluid, there were added 2,000 U of glucose dehydrogenase (product of Amano Pharmaceutical Co., Ltd.), 3 g of glucose, 2 mg of NADP and 2 g of 2-chloro-1-(3′-chlorophenyl)ethanone. This reaction mixture was stirred at 30° C. for 18 hours while adjusting the pH to 6.5 by addition of 5 M sodium hydroxide. After completion of the reaction, this reaction mixture was extracted with toluene, the solvent was then removed, and the extract was analyzed. 2-Chloro-1-(3′-chlorophenyl)ethanol was obtained in 96% yield. The 2-chloro-1-(3′-chlorophenyl)ethanol formed on that occasion was the R form with an optical purity of 99.9% ee.

WORKUP

后处理

customas obtained in Example 8
customAfter completion of the reaction
extractionthis reaction mixture was extracted with toluene
customthe solvent was then removed