Acetic Acid → Triethoxysilane

HRID 2058979Notes

Reaction equation

HRID 2058979 reaction equation
NCCC[Si:5]([O:12][CH2:13][CH3:14])([O:9][CH2:10][CH3:11])[O:6][CH2:7][CH3:8].C(O)(=O)C>O>[CH2:7]([O:6][SiH:5]([O:12][CH2:13][CH3:14])[O:9][CH2:10][CH3:11])[CH3:8]

Participants

4

Reactant 2

HCID 176Acetic AcidHCID 13521(3-Aminopropyl)triethoxysilane

Product 1

HCID 13830Triethoxysilane

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

3-Aminopropyltriethoxysilane (1 ml) and 1.8 ml of 0.1% acetic acid were made up to 10 ml with water and added to 0.4 g sedimented yeast cells, which had been produced as described in Example 1. The mixture was stirred at room temperature for 24 hours and the cells were then separated and washed thoroughly with water. A 1% suspension of the treated cells in PBS, containing 5% glutaraldehyde was added to 2.5 mg of horseradish peroxidase in 2.5 ml PBS and kept at 21° C. for one hour. The cells were then separated and washed with PBS by centrifugation.

Workup

  1. additionadded to 0.4 g
  2. customhad been produced
  3. customthe cells were then separated
  4. washwashed thoroughly with water
  5. additionA 1% suspension of the treated cells in PBS, containing 5% glutaraldehyde
  6. additionwas added to 2.5 mg of horseradish peroxidase in 2.5 ml PBS
  7. waitkept at 21° C. for one hour
  8. customThe cells were then separated
  9. washwashed with PBS by centrifugation

Sources & evidence

ORD record
ord-13467f087d494b579d5ab8dc885aaff9
Source dataset
uspto-grants-1982_03
Patent number
US04320194

Reaction notes

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